Synthesis characterization and bioactivities of chemically and biologically generated nanoparticles with special emphasis on antiproliferative potential in MCF 7 breast cancer cell line

Abstract

This study aimed to develop novel nanoparticle-basedtreatment strategies for breast cancer. For that, we used both thechemical and biological synthesis approaches; The first approachcreated an effective drug delivery system using chemically synthesizedIron oxide Gold core-shell-nanoparticles (IONPs). A bacterialmacrolide-derived compound, Rapamycin, which has already provenanti-proliferative ability in breast cancer cells, is used here to check theefficiency of the chemically synthesized nanocarrier system. In thesecond approach, the cell-free supernatant of the soil bacterial isolateswas used to synthesize Gold nanoparticles with anti-proliferativeproperties in the human breast adenocarcinoma cell line, MCF-7. Wealso characterized the AuNPs and assessed their anti-oxidant, anti-fungal, and anti-bacterial properties.The results documented the first report on the successfuldelivery of Rapamycin using the Au-IONP delivery system thatsuccessfully delivered and enhanced the anti-cancer activity ofRapamycin. And also, the first-ever production of gold nanoparticlesfrom Enterobacter hormaechei soil isolates with exceptionalanticancer and anti-bacterial potentials. This research successfullyconstituted two different methodologies for the synthesis ofnanoparticles with comparable anti-proliferative effects in breastcancer cells.