Modulation of abiotic stress signals in in vitro morphogenic responses and secondary metabolites production of ayapana triplinervis vahl r m king and h rob

Abstract

Ayapana triplinervis (Vahl) R.M.King & H.Rob, belonging to the family Asteraceae,is a medicinal plant with immense medicinal properties due to the presence of a number ofsecondary metabolites like ayapanin (7-methoxycoumarin), hydragetin, daphnetin etc. Invitro production of this plant can be a convenient method for the commercial production ofthese metabolites since this method prevents the exploitation of natural population of thisplant. The present research work revealed how abiotic stress signals affect in vitromorphogenic responses and secondary metabolites production of A. triplinervis. The entireplant culture study was conducted using MS medium in an in vitro system.The initial objective of the study was to develop protocols for direct and indirectorganogenesis of A. triplinervis using varying concentrations of plant growth regulators.Nodal explants were selected for direct organogenesis, and leaf and internodal explants wereused for indirect organogenesis and inoculated to the MS media containing differentcombinations and concentrations of Kin, BAP, NAA, IBA, and TDZ. 0.5 mg l -1 Kin wasfound to be best for the rapid multiplication of shoots from the single nodal explants (directorganogenesis) while TDZ (1.0 mg l-1 and 1.5 mg l-1) alone and TDZ (1.5 mg l-1 and 2.0 mgl-1) + NAA (0.5 mg l-1) supplemented media was found to be most effective in shootregeneration from the leaf explants (indirect organogenesis). The plants developed from theleaf explants through indirect organogenesis were subjected to genetic fidelity analysis usingISSR markers and it was found that the regenerants were genetically 100% similar to themother plant.The effect of different concentrations of various stress signals, NaCl, SA, Sucrose,MS medium strengths, Put, Trp, JA, and Chitosan, were also studied in A. triplinervis in vitrocultures. It was found that the morphogenic responses of the plant varied for each stresssignals used. The secondary metabolite profile of the plant was studied using HPTLC. TheHPTLC studies on the methanolic extracts of A. triplinervis, revealed the presence ofmaximum number of compounds in plants grown in SA supplemented media as well as in the various strenght of MS media.in the case of ‘ayapanion’ content the procuction wasenhanced in the media fortified with NaCl, Sucrose, Put and Trp compared to the controlplants. The study thus, validated the effects of several abiotic stress signals at differentconcentrations in the secondary metabolite production of Ayapana triplinervis.